Based on the identification of the bottlenecks hampering the production of proteins in E. coli, our laboratory engineers and selects protein production strains. In this project fundamental and applied research will be combined in a unique way. A host of different techniques and approaches will be used; e.g., genetics, molecular biology, flow cytometry, fluorescence microscopy, proteomics and whole genome sequencing.
The project will be carried out in collaboration with several academic and industrial partners.
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- Wagner et al. Proc. Natl. Acad. Sci. USA. 2008, 38:14371-6.
- Schlegel et al. J. Mol. Biol. 2012, 423: 648-59.
- Schlegel et al. Microb. Cell Factories 2013, doi:10.1186/1475-2859-12-24.
The Department of Biochemistry and Biophysics is mainly located with the other Departments of Chemistry and Life Sciences in the Arrhenius Laboratories for Natural Sciences, which are situated in the northern part of the University Campus at Frescati. Presently around 190 people are working at the Department of which 85 are PhD students engaged in internationally highly recognized research covering a broad range of subjects.
The Department is also deeply involved in teaching, with courses at all undergraduate levels, including a wide range of Master courses. A close link between the undergraduate program and the research projects has since long been a tradition and a trademark of the Department. Three centers are linked to the Department: Stockholm Center for Biomembrane Research, Science for Life Laboratory and Stockholm Bioinformatics Centre.
More than 20 research groups are active at the Department of Biochemistry and Biophysics. The research projects span across a broad range of topics covering various aspects of structure and function of biological systems. A majority of these topics are centered on biological membranes, where many groups working within this area are part of the Center for Biomembrane Research, which is hosted by the department. Also the Stockholm Bioinformatics Centre and the Science for Life Laboratory are closely linked to the department. The combination of the highly interdisciplinary expertise and research projects at the department is unique in Sweden and also at an international level.
This expertise ranges across cell biology, biochemistry, biophysics and theory. Some specific topics that are addressed include membrane protein biogenesis, membrane protein topology & assembly, mitochondria & chloroplasts, protein structure & function, protein folding & trafficking, lipid biosynthesis & function, energy conversion, DNA-RNA-PNA interactions, biological nitrogen fixation, viral membrane glycoproteins, protein structure & disease, bioinformatics, computational structural biology and development of theoretical tools. The experimental studies are performed in different organisms and are also combined with the use of a wide range of advanced biochemical and biophysical techniques.
